Hotspot

trnR-UCU-atpA

LSC · 12175-12687 · 513 bp

Back to Pithecellobium

Pi 0.0298

MarkerSeek score 73.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 513
variable_sites: 15
pi: 0.0298
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CATTCACTCGGGAAGCAGAA	47.6	50.0	57.3	57.9	627-643 bp	1.0	68.8
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AAGACATTCACTCGGGAAGC	47.6	50.0	57.3	57.9	631-647 bp	1.0	68.8
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	617-633 bp	1.0	66.6
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ATTCACTCGGGAAGCAGAAG	47.6	50.0	57.3	57.6	626-642 bp	1.0	66.5
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	CATTCACTCGGGAAGCAGAA	47.6	50.0	56.9	57.9	662-678 bp	1.0	65.8