Hotspot

trnQ-UUG-psbK

LSC · 8123-8802 · 680 bp

Back to Pithecellobium

Pi 0.029

MarkerSeek score 71.40

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 19
pi: 0.029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	CTTCGAATAGCGGGACCAAA	AGGCTTCAGGTAATTTCGCG	50.0	50.0	58.0	58.6	819-901 bp	1.0	78.9
trnQ-UUG-psbK_p2	CTTCGAATAGCGGGACCAAA	GGCTTCAGGTAATTTCGCGA	50.0	50.0	58.0	58.6	818-900 bp	1.0	78.9
trnQ-UUG-psbK_p3	TCGAATAGCGGGACCAAAAC	AGGCTTCAGGTAATTTCGCG	50.0	50.0	58.3	58.6	817-899 bp	1.0	76.9
trnQ-UUG-psbK_p4	TCGAATAGCGGGACCAAAAC	GGCTTCAGGTAATTTCGCGA	50.0	50.0	58.3	58.6	816-898 bp	1.0	76.9
trnQ-UUG-psbK_p5	CTTCGAATAGCGGGACCAAA	GCTTCAGGTAATTTCGCGAAG	50.0	47.6	58.0	58.0	817-899 bp	1.0	75.6