Hotspot

rps11

LSC · 74590-75006 · 417 bp

Back to Pitcairnia

Pi 0.0024

MarkerSeek score 43.90

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 417
variable_sites: 1
pi: 0.0024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	GGGTTCCATACGATATCCGC	GGATCCGGCGCAGTTTTATA	55.0	50.0	58.0	58.1	1459-1460 bp	1.0	79.7
rps11_p2	GGGTTCCATACGATATCCGC	GCGTGGGCGAATTATAGTGA	55.0	50.0	58.0	58.1	1177-1178 bp	1.0	79.7
rps11_p3	TCTAGGACCCCTTACGCAAA	GGATCCGGCGCAGTTTTATA	50.0	50.0	58.1	58.1	1318-1319 bp	1.0	79.3
rps11_p4	GGGTTCCATACGATATCCGC	ACTGAATCACTTCCCAACGG	55.0	50.0	58.0	57.8	1535-1536 bp	1.0	79.1
rps11_p5	GGGTTCCATACGATATCCGC	CTGAATCACTTCCCAACGGT	55.0	50.0	58.0	57.8	1534-1535 bp	1.0	79.1