Hotspot

rps11-rpl36

LSC · 75007-75158 · 152 bp

Back to Pitcairnia

Pi 0.0662

MarkerSeek score 78.20

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 10
pi: 0.0662
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ACGCCCATTCCTATGTGAAC	GCGTGGGCGAATTATAGTGA	50.0	50.0	58.0	58.1	254-255 bp	1.0	82.0
rps11-rpl36_p2	GAACCAATACGCCCATTCCT	GCGTGGGCGAATTATAGTGA	50.0	50.0	57.9	58.1	262-263 bp	1.0	81.8
rps11-rpl36_p3	ACGCCCATTCCTATGTGAAC	AAGGGCTTCCGTTCGTAAAA	50.0	45.0	58.0	57.7	302-303 bp	1.0	80.5
rps11-rpl36_p4	GAACCAATACGCCCATTCCT	AAGGGCTTCCGTTCGTAAAA	50.0	45.0	57.9	57.7	310-311 bp	1.0	80.3
rps11-rpl36_p5	TGAACCAATACGCCCATTCC	GCGTGGGCGAATTATAGTGA	50.0	50.0	58.2	58.1	263-264 bp	1.0	80.3