Hotspot

accD-psaI

LSC · 53050-54034 · 985 bp

Back to Pitcairnia

Pi 0.00921

MarkerSeek score 64.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 985
variable_sites: 9
pi: 0.00921
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1099-1149 bp	1.0	79.2
accD-psaI_p2	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1098-1148 bp	1.0	79.2
accD-psaI_p3	AGCTCCATGGTTTCTTTCCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	1105-1155 bp	1.0	78.6
accD-psaI_p4	GCTCCATGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	1104-1154 bp	1.0	78.6
accD-psaI_p5	GACCCAATCGTACCACGTAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	1151-1201 bp	1.0	75.7