Hotspot

trnC-GCA-petN

LSC · 20704-21755 · 1052 bp

Back to Pitcairnia

Pi 0.00775
MarkerSeek score 62.00
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1052
variable_sites
8
pi
0.00775
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 AGTTCAAATCTGGGTGTCGC AGTCCACTCCTTCCCCATAC 50.0 55.0 58.5 58.1 1140-1160 bp 1.0 77.2
trnC-GCA-petN_p2 AGTTCAAATCTGGGTGTCGC GTCCACTCCTTCCCCATACT 50.0 55.0 58.5 58.1 1139-1159 bp 1.0 77.2
trnC-GCA-petN_p3 AGTTCAAATCTGGGTGTCGC AGCCCAAGCGAGACTTACTA 50.0 50.0 58.5 58.2 1081-1101 bp 1.0 77.0
trnC-GCA-petN_p4 AGTTCAAATCTGGGTGTCGC GAGTCCACTCCTTCCCCATA 50.0 55.0 58.5 57.8 1141-1161 bp 1.0 76.7
trnC-GCA-petN_p5 AGTTCAAATCTGGGTGTCGC CTAGAGTCCACTCCTTCCCC 50.0 60.0 58.5 58.2 1144-1164 bp 1.0 76.4