Hotspot

rpoB-trnC-GCA

LSC · 19317-20632 · 1316 bp

Back to Pitcairnia

Pi 0.00533
MarkerSeek score 62.00
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1316
variable_sites
7
pi
0.00533
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TCCCTCATTTCCATTCCGGA GCGACACCCAGATTTGAACT 50.0 50.0 58.4 58.5 1466 bp 1.0 73.4
rpoB-trnC-GCA_p2 TTCCCTCATTTCCATTCCGG GCGACACCCAGATTTGAACT 50.0 50.0 57.6 58.5 1467 bp 1.0 73.2
rpoB-trnC-GCA_p3 TCCCTCATTTCCATTCCGGA TTTGTTGATCAGGCGACACC 50.0 50.0 58.4 58.8 1478 bp 1.0 71.1
rpoB-trnC-GCA_p4 TTCCCTCATTTCCATTCCGG TTTGTTGATCAGGCGACACC 50.0 50.0 57.6 58.8 1479 bp 1.0 70.9
rpoB-trnC-GCA_p5 TCCCTCATTTCCATTCCGGA TTTTGTTGATCAGGCGACAC 50.0 45.0 58.4 56.9 1479 bp 1.0 68.8