Hotspot

atpH-atpI

LSC · 6026-6987 · 962 bp

Back to Pitcairnia

Pi 0.0177

MarkerSeek score 69.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 962
variable_sites: 17
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1019-1075 bp	1.0	79.7
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	1075-1131 bp	1.0	79.0
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	45.0	55.0	57.9	57.8	1035-1091 bp	1.0	78.8
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1020-1076 bp	1.0	78.5
atpH-atpI_p5	GAAGCAAGTCCTACAGCCAA	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	1049-1105 bp	1.0	78.4