Hotspot

rpoA

LSC · 82145-83131 · 987 bp

Back to Pistacia

Pi 0.00116

MarkerSeek score 34.20

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 987
variable_sites: 3
pi: 0.00116
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TAATGGTTTCAGTACCCGCG	GGTTCACGTAAAAGTGCACG	50.0	50.0	58.0	58.0	1883-1888 bp	1.0	81.1
rpoA_p2	TAATGGTTTCAGTACCCGCG	AGGGGTACAAGAAGAGGGAC	50.0	55.0	58.0	58.0	1748-1753 bp	1.0	80.9
rpoA_p3	TAATGGTTTCAGTACCCGCG	CAGGGGTACAAGAAGAGGGA	50.0	55.0	58.0	58.0	1749-1754 bp	1.0	80.9
rpoA_p4	TAATGGTTTCAGTACCCGCG	TCAGGGGTACAAGAAGAGGG	50.0	55.0	58.0	58.0	1750-1755 bp	1.0	80.9
rpoA_p5	TAATGGTTTCAGTACCCGCG	TAGGACTGGACGGATTGGTT	50.0	50.0	58.0	58.1	1899-1904 bp	1.0	80.8