Hotspot

ccsA

SSC · 119895-120863 · 969 bp

Back to Pistacia

Pi 0.00364

MarkerSeek score 53.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 11
pi: 0.00364
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CCGTATACTTGAAAGACTCTCCC	TTGCGCTGTCCTATGTTTGT	47.8	45.0	58.1	58.1	1097 bp	1.0	55.4
ccsA_p2	ACTTCTGATTTCCATTCCGT	TTGCGCTGTCCTATGTTTGT	40.0	45.0	54.7	58.1	1113 bp	1.0	53.1
ccsA_p3	CCGTATACTTGAAAGACTCTCCC	CGGCAGCTTCCACTAAGTTT	47.8	50.0	58.1	58.5	1128-1141 bp	1.0	52.4
ccsA_p4	AACTTCTGATTTCCATTCCGT	TTGCGCTGTCCTATGTTTGT	38.1	45.0	55.5	58.1	1114 bp	1.0	51.8
ccsA_p5	CCGTATACTTGAAAGACTCTCCC	TGCGCTGTCCTATGTTTGTA	47.8	45.0	58.1	57.2	1096 bp	1.0	50.3