Hotspot

atpF

LSC · 13396-14707 · 1312 bp

Back to Pistacia

Pi 0.00276

MarkerSeek score 43.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1312
variable_sites: 11
pi: 0.00276
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTGCTCAATACGTTCACGGA	AGTCTATCTAAAAGAGGAGATCA	45.0	34.8	57.6	53.5	1450-1470 bp	1.0	40.7
atpF_p2	TGCTCAATACGTTCACGGAT	AGTCTATCTAAAAGAGGAGATCA	45.0	34.8	57.3	53.5	1449-1469 bp	1.0	40.7
atpF_p3	TTGCTCAATACGTTCACGGA	AGTCTATCTAAAAGAGGAGATCAT	45.0	33.3	57.6	54.1	1450-1470 bp	1.0	40.7
atpF_p4	ATTGCTCAATACGTTCACGGA	AGTCTATCTAAAAGAGGAGATCA	42.9	34.8	58.0	53.5	1451-1471 bp	1.0	40.7
atpF_p5	TTGCTCAATACGTTCACGGAT	AGTCTATCTAAAAGAGGAGATCA	42.9	34.8	58.0	53.5	1450-1470 bp	1.0	40.7