Hotspot

trnF-GAA-ndhJ

LSC · 51644-52412 · 769 bp

Back to Pistacia

Pi 0.00163

MarkerSeek score 62.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 4
pi: 0.00163
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GCCTGAAAGTTGGATCGGAT	55.0	50.0	58.0	58.0	825-919 bp	1.0	84.0
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	ATCCACGGCTGAAACGTATC	55.0	50.0	58.0	58.1	850-944 bp	1.0	83.8
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GCCTGAAAGTTGGATCGGAT	50.0	50.0	58.1	58.0	789-883 bp	1.0	83.6
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATCCACGGCTGAAACGTATC	50.0	50.0	58.1	58.1	814-908 bp	1.0	83.4
trnF-GAA-ndhJ_p5	ATGATGCGTCGGTAATGGTC	GCCTGAAAGTTGGATCGGAT	50.0	50.0	58.1	58.0	845-939 bp	1.0	82.9