Hotspot

trnT-GGU-psbD

LSC · 34339-35896 · 1558 bp

Back to Pistacia

Pi 0.00208

MarkerSeek score 61.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1558
variable_sites: 10
pi: 0.00208
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1701-1708 bp	1.0	78.7
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1704-1711 bp	1.0	78.5
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CCCTTCGTAACCAGTCATCC	55.0	55.0	58.1	57.7	1685-1692 bp	1.0	78.2
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CCCTTCGTAACCAGTCATCC	50.0	55.0	58.1	57.7	1688-1695 bp	1.0	78.0
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1678-1685 bp	1.0	77.0