Hotspot

rps16-trnQ-UUG

LSC · 6947-8460 · 1514 bp

Back to Pistacia

Pi 0.00333
MarkerSeek score 53.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1514
variable_sites
14
pi
0.00333
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1645-1664 bp 1.0 79.5
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAAATTGAAATGGGGCGTGG 50.0 50.0 58.2 58.0 1656-1675 bp 1.0 79.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1587-1606 bp 1.0 79.0
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1598-1617 bp 1.0 78.5
rps16-trnQ-UUG_p5 CCACAGCTGATCATGTCCTT GAAATTGAAATGGGGCGTGG 50.0 50.0 57.6 58.0 1666-1685 bp 1.0 77.3