Hotspot

trnK-UUU-rps16

LSC · 4680-5791 · 1112 bp

Back to Pistacia

Pi 0.00424

MarkerSeek score 64.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 14
pi: 0.00424
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GTATGGACGAACCCTTTGCT	TATTTCAAAGAAGGCGGGGG	50.0	50.0	57.9	57.9	1238-1262 bp	1.0	80.6
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.2	57.9	1183-1207 bp	1.0	80.2
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.2	57.9	1182-1206 bp	1.0	80.2
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	TATTTCAAAGAAGGCGGGGG	50.0	50.0	58.3	57.9	1192-1216 bp	1.0	79.3
trnK-UUU-rps16_p5	ACTCTCCCGATGGTATGGAC	TATTTCAAAGAAGGCGGGGG	55.0	50.0	58.3	57.9	1250-1274 bp	1.0	79.1