Hotspot

psbA

LSC · 268-1329 · 1062 bp

Back to Piptanthus

Pi 0

MarkerSeek score 28.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGCTCCTTTACTTTTAAAAACTCGT	AGAATTCGTGTGCTTGGGAG	32.0	50.0	57.7	58.2	1158 bp	1.0	40.0
psbA_p2	TGCTCCTTTACTTTTAAAAACTCGT	AGAGAATTCGTGTGCTTGGG	32.0	50.0	57.7	58.2	1160 bp	1.0	40.0
psbA_p3	TGCTCCTTTACTTTTAAAAACTCGT	GAGAATTCGTGTGCTTGGGA	32.0	50.0	57.7	58.2	1159 bp	1.0	40.0
psbA_p4	TGCTCCTTTACTTTTAAAAACTCGT	AATTCGTGTGCTTGGGAGTC	32.0	50.0	57.7	58.5	1156 bp	1.0	40.0
psbA_p5	TGCTCCTTTACTTTTAAAAACTCGT	GAATTCGTGTGCTTGGGAGT	32.0	50.0	57.7	58.5	1157 bp	1.0	40.0