Hotspot

ndhF

SSC · 108109-110406 · 2298 bp

Back to Piptanthus

Pi 0.00439

MarkerSeek score 60.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2298
variable_sites: 10
pi: 0.00439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATGCTATTTGGGCAGAGCAC	ACAAAAGAGATAAAACCGGCG	50.0	42.9	58.6	57.2	2412-2430 bp	1.0	61.6
ndhF_p2	ATGCTATTTGGGCAGAGCAC	CAAAAGAGATAAAACCGGCGA	50.0	42.9	58.6	56.9	2411-2429 bp	1.0	59.4
ndhF_p3	ATGCTATTTGGGCAGAGCAC	AAACCGGCGAATAAGAAACA	50.0	40.0	58.6	56.0	2418 bp	0.5	38.9
ndhF_p4	ATGCTATTTGGGCAGAGCAC	AACCGGCGAATAAGAAACAA	50.0	40.0	58.6	56.0	2417 bp	0.5	38.9
ndhF_p5	ATGCTATTTGGGCAGAGCAC	ACCGGCGAATAAGAAACAAA	50.0	40.0	58.6	56.0	2416 bp	0.5	38.9