Hotspot

ndhA

SSC · 117105-119397 · 2293 bp

Back to Piptanthus

Pi 0.00527

MarkerSeek score 60.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2293
variable_sites: 12
pi: 0.00527
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCTGTTACCATAAGGAACA	TCCGCCGGGTTTTATCAATT	42.9	45.0	56.3	57.9	2495-2512 bp	1.0	57.9
ndhA_p2	AACCCTGTTACCATAAGGAACA	TCCGCCGGGTTTTATCAATT	40.9	45.0	57.0	57.9	2496-2513 bp	1.0	55.9
ndhA_p3	TGAACCCTGTTACCATAAGGA	TCCGCCGGGTTTTATCAATT	42.9	45.0	56.0	57.9	2498-2515 bp	1.0	55.6
ndhA_p4	GAACCCTGTTACCATAAGGAACA	TCCGCCGGGTTTTATCAATT	43.5	45.0	58.1	57.9	2497-2514 bp	1.0	55.2
ndhA_p5	TGAACCCTGTTACCATAAGGAAC	TCCGCCGGGTTTTATCAATT	43.5	45.0	58.1	57.9	2498-2515 bp	1.0	55.2