Hotspot

ccsA

SSC · 111711-112691 · 981 bp

Back to Piptanthus

Pi 0.00306

MarkerSeek score 47.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 981
variable_sites: 3
pi: 0.00306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGACACGCTGCTCTTAGGA	TTTTTGATTCTGGGCCACGA	50.0	45.0	57.9	58.0	1604-1608 bp	1.0	80.4
ccsA_p2	TAAGCCGCCATGGTGAAATT	TTTTTGATTCTGGGCCACGA	45.0	45.0	58.2	58.0	1626-1630 bp	1.0	80.1
ccsA_p3	AAATTGGTAGACACGCTGCT	TTTTTGATTCTGGGCCACGA	45.0	45.0	57.8	58.0	1611-1615 bp	1.0	79.9
ccsA_p4	TGATTTCAAGTAAGCCGCCA	TTTTTGATTCTGGGCCACGA	45.0	45.0	57.8	58.0	1636-1640 bp	1.0	79.8
ccsA_p5	GTAGACACGCTGCTCTTAGG	TTTTTGATTCTGGGCCACGA	55.0	45.0	57.8	58.0	1605-1609 bp	1.0	79.8