Hotspot

trnN-GUU-trnR-ACG

IRa · 127162-127795 · 634 bp

Back to Piptanthus

Pi 0.00631

MarkerSeek score 62.40

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 634
variable_sites: 4
pi: 0.00631
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	775 bp	1.0	78.7
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	812 bp	1.0	78.1
trnN-GUU-trnR-ACG_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	816 bp	1.0	77.9
trnN-GUU-trnR-ACG_p4	AGCTCGAATTTCCCGTTGTT	CAGAGGATTAGAGCACGTGG	45.0	55.0	58.1	57.8	787 bp	1.0	77.8
trnN-GUU-trnR-ACG_p5	CCGTTGTTCCTCAGTAGCTC	TTTTAGGGTACTCCGGGGAA	55.0	50.0	58.0	57.7	829 bp	1.0	77.6