Hotspot

rps15-ycf1

SSC · 120962-121436 · 475 bp

Back to Piptanthus

Pi 0.0112

MarkerSeek score 67.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 475
variable_sites: 5
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCCGGGTTTTCTTCCTTCTT	GCCGTTTCAGTATAGTGAGGA	50.0	47.6	57.7	57.2	563-590 bp	1.0	68.0
rps15-ycf1_p2	CCCGGGTTTTCTTCCTTCTT	AGCCGTTTCAGTATAGTGAGG	50.0	47.6	57.7	57.2	564-591 bp	1.0	68.0
rps15-ycf1_p3	ATCCCGGGTTTTCTTCCTTC	GCCGTTTCAGTATAGTGAGGA	50.0	47.6	57.5	57.2	565-592 bp	1.0	66.3
rps15-ycf1_p4	GATCCCGGGTTTTCTTCCTT	GCCGTTTCAGTATAGTGAGGA	50.0	47.6	57.5	57.2	566-593 bp	1.0	66.3
rps15-ycf1_p5	ATCCCGGGTTTTCTTCCTTC	AGCCGTTTCAGTATAGTGAGG	50.0	47.6	57.5	57.2	566-593 bp	1.0	66.2