Hotspot

rps3-rps19

LSC · 81224-81776 · 553 bp

Back to Piptanthus

Pi 0.00734

MarkerSeek score 63.40

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 553
variable_sites: 4
pi: 0.00734
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CAAGTCTGAAACCAAGCGGA	CCCACTCTAAACTTCCGAGG	50.0	55.0	58.4	57.3	644-647 bp	1.0	72.8
rps3-rps19_p2	AAGTCTGAAACCAAGCGGAT	CCCACTCTAAACTTCCGAGG	45.0	55.0	57.4	57.3	643-646 bp	1.0	71.5
rps3-rps19_p3	AGTCTGAAACCAAGCGGATT	CCCACTCTAAACTTCCGAGG	45.0	55.0	57.4	57.3	642-645 bp	1.0	71.5
rps3-rps19_p4	ACCAAGTCTGAAACCAAGCG	CCCACTCTAAACTTCCGAGG	50.0	55.0	58.7	57.3	646-649 bp	1.0	70.6
rps3-rps19_p5	CAAGTCTGAAACCAAGCGGA	CACCCACTCTAAACTTCCGAG	50.0	52.4	58.4	58.0	646-649 bp	1.0	69.9