Hotspot

psaA-ycf3

LSC · 49325-50092 · 768 bp

Back to Piptanthus

Pi 0.00397

MarkerSeek score 62.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 768
variable_sites: 3
pi: 0.00397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGCCCATTCCTCGAAAGAAG	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	904-916 bp	1.0	80.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	903-915 bp	1.0	80.5
psaA-ycf3_p3	TGCCCATTCCTCGAAAGAAG	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	910-922 bp	1.0	80.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	909-921 bp	1.0	80.2
psaA-ycf3_p5	TGCCCATTCCTCGAAAGAAG	TTGGTTGAAGATCACAGGGC	50.0	50.0	57.9	58.1	868-880 bp	1.0	79.8