Hotspot

trnG-UCC-trnfM-CAU

LSC · 44030-44152 · 123 bp

Back to Piptanthus

Pi 0.025

MarkerSeek score 66.30

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 3
pi: 0.025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	201-211 bp	1.0	80.7
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	237-247 bp	1.0	80.4
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	195-205 bp	1.0	80.2
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	50.0	50.0	57.8	57.8	194-204 bp	1.0	79.6
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	199-209 bp	1.0	79.6