Hotspot

trnS-UGA-lhbA

LSC · 43050-43416 · 367 bp

Back to Piptanthus

Pi 0.00272

MarkerSeek score 49.80

Primer available yes

Label trnS-UGA-lhbA

Feature Metadata

label_name: trnS-UGA-lhbA
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 1
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-lhbA_p1	CGTCGACCAAAAGGAGAGAG	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	570-571 bp	1.0	78.4
trnS-UGA-lhbA_p2	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	487-488 bp	1.0	78.4
trnS-UGA-lhbA_p3	GAGCTATCAACCACTCAGCC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	494-495 bp	1.0	78.1
trnS-UGA-lhbA_p4	GGAGCTATCAACCACTCAGC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	495-496 bp	1.0	78.1
trnS-UGA-lhbA_p5	CAAGACCGGAGCTATCAACC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	502-503 bp	1.0	78.0