Hotspot

psbC-trnS-UGA

LSC · 42768-42956 · 189 bp

Back to Piptanthus

Pi 0.0688

MarkerSeek score 83.60

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 189
variable_sites: 13
pi: 0.0688
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCATTTATGGCACGCAGGAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	57.9	58.0	384 bp	1.0	81.6
psbC-trnS-UGA_p2	TCATTTATGGCACGCAGGAA	GCTGAGTGGTTGATAGCTCC	45.0	55.0	57.9	58.1	376 bp	1.0	81.4
psbC-trnS-UGA_p3	TCATTTATGGCACGCAGGAA	GGCTGAGTGGTTGATAGCTC	45.0	55.0	57.9	58.1	377 bp	1.0	81.4
psbC-trnS-UGA_p4	TCATTTATGGCACGCAGGAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.9	58.1	369 bp	1.0	81.4
psbC-trnS-UGA_p5	TTTATGGCACGCAGGAAGAG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.3	58.0	381 bp	1.0	80.5