Hotspot

trnD-GUC-trnY-GUA

LSC · 37579-38055 · 477 bp

Back to Piptanthus

Pi 0.0126

MarkerSeek score 51.60

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 477
variable_sites: 6
pi: 0.0126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	TATCTATCGGGACTGACGGG	CTGGTTCAAATCCAGCTCGG	55.0	55.0	57.8	58.9	581-582 bp	1.0	72.3
trnD-GUC-trnY-GUA_p2	TATCTATCGGGACTGACGGG	TCTACGCTGGTTCAAATCCA	55.0	45.0	57.8	56.9	587-588 bp	1.0	70.4
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	CTGGTTCAAATCCAGCTCGG	50.0	55.0	58.5	58.9	530-531 bp	1.0	70.1
trnD-GUC-trnY-GUA_p4	TATCTATCGGGACTGACGGG	TGGTTCAAATCCAGCTCGG	55.0	52.6	57.8	57.8	580-581 bp	1.0	69.6
trnD-GUC-trnY-GUA_p5	TATCTATCGGGACTGACGGG	GTCTACGCTGGTTCAAATCCA	55.0	47.6	57.8	58.3	588-589 bp	1.0	69.1