Hotspot

ndhJ-trnC-GCA

LSC · 12302-13292 · 991 bp

Back to Piptanthus

Pi 0.00578

MarkerSeek score 59.60

Primer available yes

Label ndhJ-trnC-GCA

Feature Metadata

label_name: ndhJ-trnC-GCA
feature_type: igs
region: LSC
length_bp: 991
variable_sites: 4
pi: 0.00578
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnC-GCA_p1	TTGGATAGGATGGCCTTTGC	ATTTGTATCGTTTTGGCGGC	50.0	45.0	57.9	57.7	850-1149 bp	1.0	81.6
ndhJ-trnC-GCA_p2	TTGGATAGGATGGCCTTTGC	AGTTCAAATCTGGGTGTCGC	50.0	50.0	57.9	58.5	787-1086 bp	1.0	80.0
ndhJ-trnC-GCA_p3	TAGGATGGCCTTTGCGTAAG	ATTTGTATCGTTTTGGCGGC	50.0	45.0	57.7	57.7	845-1144 bp	1.0	79.5
ndhJ-trnC-GCA_p4	ATGCCCGAAAGTTGGATAGG	ATTTGTATCGTTTTGGCGGC	50.0	45.0	57.7	57.7	861-1160 bp	1.0	79.4
ndhJ-trnC-GCA_p5	TAGGATGGCCTTTGCGTAAG	AGTTCAAATCTGGGTGTCGC	50.0	50.0	57.7	58.5	782-1081 bp	1.0	77.9