Hotspot

rps15

SSC · 131056-131328 · 273 bp

Back to Piptadenia

Pi 0.478

MarkerSeek score 76.60

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 120
pi: 0.478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	ACTCCCTCAACCCGTTCTAA	GCAGCCGTTTCAGTATGGTA	50.0	50.0	58.0	58.0	1456 bp	0.5	59.7
rps15_p2	CAATGCCTACTCCCTCAACC	GCAGCCGTTTCAGTATGGTA	55.0	50.0	58.0	58.0	1464 bp	0.5	59.5
rps15_p3	CCAACAATGCCTACTCCCTC	GCAGCCGTTTCAGTATGGTA	55.0	50.0	58.0	58.0	1468 bp	0.5	59.5
rps15_p4	TCCTAACTGTTCCGGTCCAT	GCAGCCGTTTCAGTATGGTA	50.0	50.0	58.1	58.0	1109 bp	0.5	59.4
rps15_p5	TGCATTGATGGATGGTGAGG	GCAGCCGTTTCAGTATGGTA	50.0	50.0	57.9	58.0	907 bp	0.5	59.4