Hotspot

psaC

SSC · 124834-125079 · 246 bp

Back to Piptadenia

Pi 0.5

MarkerSeek score 77.20

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 117
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TTTCTTTCCACCCCACATGG	TGATCGTGTTTGCGAAAACG	50.0	45.0	58.0	58.0	1031 bp	0.5	59.8
psaC_p2	TTTCTTTCCACCCCACATGG	GATCGTGTTTGCGAAAACGT	50.0	45.0	58.0	58.0	1030 bp	0.5	59.8
psaC_p3	TTTCTTTCCACCCCACATGG	ACGTATGATCGTGTTTGCGA	50.0	45.0	58.0	58.0	1036 bp	0.5	59.8
psaC_p4	CCCGTCAGTAAAATAGGGCC	TGATCGTGTTTGCGAAAACG	55.0	45.0	58.0	58.0	835 bp	0.5	59.6
psaC_p5	CCCGTCAGTAAAATAGGGCC	GATCGTGTTTGCGAAAACGT	55.0	45.0	58.0	58.0	834 bp	0.5	59.6