Hotspot

ndhI

SSC · 126655-127140 · 486 bp

Back to Piptadenia

Pi 0.467

MarkerSeek score 77.40

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 486
variable_sites: 184
pi: 0.467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	GTGCTGCAGCTACAAAATGG	GGGGATGGAATTTATGGCGT	50.0	50.0	58.0	58.0	1471 bp	0.5	59.9
ndhI_p2	GTGCTGCAGCTACAAAATGG	TGGGGATGGAATTTATGGCG	50.0	50.0	58.0	58.0	1472 bp	0.5	59.8
ndhI_p3	CAAGAACCAGTCCCAACGAA	GGGGATGGAATTTATGGCGT	50.0	50.0	58.0	58.0	1417 bp	0.5	59.7
ndhI_p4	CAAGAACCAGTCCCAACGAA	TGGGGATGGAATTTATGGCG	50.0	50.0	58.0	58.0	1418 bp	0.5	59.6
ndhI_p5	TCCCAACGAAAAGGCAGAAA	GGGGATGGAATTTATGGCGT	45.0	50.0	57.9	58.0	1407 bp	0.5	59.5