Hotspot

ndhG

SSC · 125885-126415 · 531 bp

Back to Piptadenia

Pi 0.472

MarkerSeek score 80.60

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 242
pi: 0.472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CGTTTTCGCAAACACGATCA	GATTACCTTAGCCCACGCAA	45.0	50.0	58.0	58.0	1819 bp	0.5	59.6
ndhG_p2	CGTTTTCGCAAACACGATCA	TACCTTAGCCCACGCAAATC	45.0	50.0	58.0	58.0	1816 bp	0.5	59.6
ndhG_p3	CGTTTTCGCAAACACGATCA	AACAAACAGTACGAGCTGCA	45.0	45.0	58.0	58.1	1862 bp	0.5	59.5
ndhG_p4	CGTTTTCGCAAACACGATCA	ACAAACAGTACGAGCTGCAA	45.0	45.0	58.0	58.1	1861 bp	0.5	59.5
ndhG_p5	CGTTTTCGCAAACACGATCA	AATCAAATTGCTTTGGGCCG	45.0	45.0	58.0	57.9	1488 bp	0.5	59.1