Hotspot

ndhA

SSC · 127221-129769 · 2549 bp

Back to Piptadenia

Pi 0.475

MarkerSeek score 76.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2549
variable_sites: 954
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GATTTGCGTGGGCTAAGGTA	GTGAGAGTTGAAGCCCCAAA	50.0	50.0	58.0	58.0	2934 bp	0.5	59.6
ndhA_p2	TTGCGTGGGCTAAGGTAATC	GTGAGAGTTGAAGCCCCAAA	50.0	50.0	58.0	58.0	2931 bp	0.5	59.6
ndhA_p3	TGCAGCTCGTACTGTTTGTT	GTGAGAGTTGAAGCCCCAAA	45.0	50.0	58.1	58.0	2888 bp	0.5	59.4
ndhA_p4	TTGCAGCTCGTACTGTTTGT	GTGAGAGTTGAAGCCCCAAA	45.0	50.0	58.1	58.0	2889 bp	0.5	59.4
ndhA_p5	ACCAATGTACCTTGCAGCTC	GTGAGAGTTGAAGCCCCAAA	50.0	50.0	58.2	58.0	2900 bp	0.5	58.5