Hotspot

trnL-UAG-ccsA

SSC · 121543-121631 · 89 bp

Back to Piptadenia

Pi 0.456

MarkerSeek score 76.20

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 89
variable_sites: 36
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	GCTGCATAGCCCAATACCAT	50.0	50.0	58.0	58.1	612 bp	0.5	59.3
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	GCTGCATAGCCCAATACCAT	50.0	50.0	58.0	58.1	611 bp	0.5	59.2
trnL-UAG-ccsA_p3	GTGAAATCGGTAGACACGCT	ATCATGCCTTTTTCGGACGA	50.0	45.0	58.0	57.9	297 bp	0.5	59.1
trnL-UAG-ccsA_p4	TGAAATCGGTAGACACGCTG	ATCATGCCTTTTTCGGACGA	50.0	45.0	58.0	57.9	296 bp	0.5	59.1
trnL-UAG-ccsA_p5	GTGAAATCGGTAGACACGCT	TGCCTTTTTCGGACGAATCA	50.0	45.0	58.0	58.1	293 bp	0.5	59.0