Hotspot

rpl32-trnL-UAG

SSC · 121005-121462 · 458 bp

Back to Piptadenia

Pi 0.491

MarkerSeek score 77.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 458
variable_sites: 192
pi: 0.491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACGACCAAGTAACCGGGATA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	999 bp	0.5	58.7
rpl32-trnL-UAG_p2	ACGACCAAGTAACCGGGATA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	1000 bp	0.5	58.7
rpl32-trnL-UAG_p3	ACGACCAAGTAACCGGGATA	GCTGCATAGCCCAATACCAT	50.0	50.0	58.1	58.1	1591 bp	0.5	58.1
rpl32-trnL-UAG_p4	ACGACCAAGTAACCGGGATA	ATCATGCCTTTTTCGGACGA	50.0	45.0	58.1	57.9	1276 bp	0.5	57.9
rpl32-trnL-UAG_p5	ACGACCAAGTAACCGGGATA	TGCCTTTTTCGGACGAATCA	50.0	45.0	58.1	58.1	1272 bp	0.5	57.9