Hotspot

ndhF-rpl32

SSC · 119883-120836 · 954 bp

Back to Piptadenia

Pi 0.424

MarkerSeek score 79.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 954
variable_sites: 326
pi: 0.424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGGGAATTGGGCAGACTTAG	AGCGTGTCTACCGATTTCAC	55.0	50.0	57.9	58.0	2344 bp	0.5	59.5
ndhF-rpl32_p2	GGGGAATTGGGCAGACTTAG	CAGCGTGTCTACCGATTTCA	55.0	50.0	57.9	58.0	2345 bp	0.5	59.5
ndhF-rpl32_p3	AAATAACAGGAAGGCGCACA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	2311 bp	0.5	59.2
ndhF-rpl32_p4	AATAACAGGAAGGCGCACAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	2310 bp	0.5	59.2
ndhF-rpl32_p5	ATAACAGGAAGGCGCACAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	2309 bp	0.5	59.2