Hotspot

ndhG-ndhE

SSC · 124334-124564 · 231 bp

Pi 0.0547

MarkerSeek score 68.90

Primer available yes

Label ndhG-ndhE

Feature Metadata

label_name: ndhG-ndhE
feature_type: igs
region: SSC
length_bp: 231
variable_sites: 28
pi: 0.0547
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhE_p1	TGGGGATGGGGTTACTTCAT	ACATCCGTCCCAAGGTATCA	50.0	50.0	58.0	58.1	1190-1213 bp	1.0	83.3
ndhG-ndhE_p2	TGGGGATGGGGTTACTTCAT	ATCATTTCCAACACGTCCGT	50.0	45.0	58.0	57.8	1174-1197 bp	1.0	82.9
ndhG-ndhE_p3	TGGGGATGGGGTTACTTCAT	CGAGTTTTTGGGCGTTTTCA	50.0	45.0	58.0	57.8	1079-1080 bp	0.285714	49.9
ndhG-ndhE_p4	CTGCATGAATTCGCATTGCA	ACATCCGTCCCAAGGTATCA	45.0	50.0	58.1	58.1	1617-1618 bp	0.285714	49.8
ndhG-ndhE_p5	CTGCATGAATTCGCATTGCA	ATCATTTCCAACACGTCCGT	45.0	45.0	58.1	57.8	1601-1602 bp	0.285714	49.4