Hotspot

ndhC-trnV-UAC

LSC · 52385-53729 · 1345 bp

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Pi 0.0251
MarkerSeek score 70.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1345
variable_sites
77
pi
0.0251
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAACACTTGA AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.8 57.8 1449-1594 bp 1.0 46.1
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAACACTTGA GAAGGTCTACGGTTCGAGTC 33.3 55.0 56.8 57.5 1448-1593 bp 1.0 46.1
ndhC-trnV-UAC_p3 TGCCAAAATAGGAATAACACTTGA GAGCCCAATGCAAAGCAAAA 33.3 45.0 56.8 58.1 1537-1685 bp 1.0 45.8
ndhC-trnV-UAC_p4 TGCCAAAATAGGAATAACACTTGA AGCCCAATGCAAAGCAAAAA 33.3 40.0 56.8 57.7 1536-1684 bp 1.0 45.8
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAACACTTG GAGCCCAATGCAAAGCAAAA 34.8 45.0 55.5 58.1 1537-1685 bp 1.0 45.8