Hotspot

psbZ-trnG-GCC

LSC · 37804-38091 · 288 bp

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Pi 0.0385
MarkerSeek score 72.40
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
288
variable_sites
26
pi
0.0385
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 AGTGGGTATCCTTAATTCTCTCA AATCGAACCCGCATCTTCTC 39.1 50.0 56.2 58.1 377-379 bp 1.0 47.9
psbZ-trnG-GCC_p2 AGTGGGTATCCTTAATTCTCTCA GAATCGAACCCGCATCTTCT 39.1 50.0 56.2 58.1 378-380 bp 1.0 47.8
psbZ-trnG-GCC_p3 GTGGGTATCCTTAATTCTCTCA AATCGAACCCGCATCTTCTC 40.9 50.0 54.7 58.1 376-378 bp 1.0 47.0
psbZ-trnG-GCC_p4 TGGATTAGTTTTTCTAGTGGGT AATCGAACCCGCATCTTCTC 36.4 50.0 54.7 58.1 392-394 bp 1.0 46.7
psbZ-trnG-GCC_p5 TGGATTAGTTTTTCTAGTGGGT GAATCGAACCCGCATCTTCT 36.4 50.0 54.7 58.1 393-395 bp 1.0 46.7