Hotspot

trnQ-UUG-psbK

LSC · 7418-7741 · 324 bp

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Pi 0.0344
MarkerSeek score 68.40
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
324
variable_sites
26
pi
0.0344
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 GAAGGATTCGAACCTCCGAG ACTCGAATGAAGGGCAGAAC 55.0 50.0 57.8 57.9 439 bp 1.0 82.2
trnQ-UUG-psbK_p2 GAAGGATTCGAACCTCCGAG AAGCTACTCGAATGAAGGGC 55.0 50.0 57.8 57.7 444 bp 1.0 80.5
trnQ-UUG-psbK_p3 AGGATTCGAACCTCCGAGTA ACTCGAATGAAGGGCAGAAC 50.0 50.0 57.6 57.9 437 bp 1.0 80.5
trnQ-UUG-psbK_p4 AAGGATTCGAACCTCCGAGT ACTCGAATGAAGGGCAGAAC 50.0 50.0 58.4 57.9 438 bp 1.0 80.4
trnQ-UUG-psbK_p5 GAGTAACGGGACCAAAACCC ACTCGAATGAAGGGCAGAAC 55.0 50.0 58.5 57.9 422 bp 1.0 80.3