Hotspot

rps16-trnQ-UUG

LSC · 6391-7344 · 954 bp

Back to Piper

Pi 0.0335
MarkerSeek score 76.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
954
variable_sites
67
pi
0.0335
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1023-1318 bp 1.0 85.3
rps16-trnQ-UUG_p2 TGATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1034-1329 bp 1.0 84.7
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1027-1322 bp 1.0 84.2
rps16-trnQ-UUG_p4 TGATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 1038-1333 bp 1.0 83.6
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA ACATATAAATGGGGCGTGGC 45.0 50.0 58.1 58.4 1080-1375 bp 1.0 82.5