Hotspot

trnK-UUU-rps16

LSC · 4294-5289 · 996 bp

Back to Piper

Pi 0.036
MarkerSeek score 79.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
996
variable_sites
81
pi
0.036
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1139-1182 bp 1.0 85.0
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1138-1181 bp 1.0 85.0
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.5 58.2 1125-1168 bp 1.0 82.8
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG GGTGCTCAACCTACAGGAAC 50.0 55.0 58.6 58.2 1150-1193 bp 1.0 81.7
trnK-UUU-rps16_p5 TCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 45.0 55.0 57.3 58.2 1148-1191 bp 1.0 80.5