Hotspot

trnH-GUG-psbA

LSC · 102-293 · 192 bp

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Pi 0.0742
MarkerSeek score 68.00
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
192
variable_sites
30
pi
0.0742
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CAATCCACTGCCTTGATCCA AACCGTGCAAACCTTGGTAT 50.0 45.0 57.9 58.0 327-330 bp 1.0 86.9
trnH-GUG-psbA_p2 AATCCACTGCCTTGATCCAC AACCGTGCAAACCTTGGTAT 50.0 45.0 57.9 58.0 326-329 bp 1.0 86.8
trnH-GUG-psbA_p3 ACAATCCACTGCCTTGATCC AACCGTGCAAACCTTGGTAT 50.0 45.0 57.9 58.0 328-331 bp 1.0 86.8
trnH-GUG-psbA_p4 CTTGATCCACTTGGCCACAT AACCGTGCAAACCTTGGTAT 50.0 45.0 58.2 58.0 316-319 bp 1.0 86.7
trnH-GUG-psbA_p5 CAATCCACTGCCTTGATCCA GCGTAATGCACACAACTTCC 50.0 50.0 57.9 58.0 292-295 bp 1.0 86.6