Hotspot

rps18

Genome · 31851-32153 · 303 bp

Pi 0.00495

MarkerSeek score 34.30

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: Genome
length_bp: 303
variable_sites: 3
pi: 0.00495
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	GCAGTAGCAAAAGCCATTGG	TATGGCCAAGAGCGGAGATA	50.0	50.0	58.0	58.0	1369 bp	1.0	80.2
rps18_p2	GCAGTAGCAAAAGCCATTGG	TGGCCAAGAGCGGAGATATA	50.0	50.0	58.0	58.0	1367 bp	1.0	80.2
rps18_p3	AAGAGGCAGTTGCTTCCAAA	TATGGCCAAGAGCGGAGATA	45.0	50.0	57.9	58.0	992 bp	1.0	79.7
rps18_p4	AAGAGGCAGTTGCTTCCAAA	TGGCCAAGAGCGGAGATATA	45.0	50.0	57.9	58.0	990 bp	1.0	79.7
rps18_p5	GCAGTAGCAAAAGCCATTGG	GGACTGGAAGGGGGTAGAAT	50.0	55.0	58.0	58.1	1579 bp	1.0	79.5