Hotspot

psaM

Genome · 8299-8391 · 93 bp

Pi 0.0394

MarkerSeek score 59.00

Primer available yes

Label psaM

Feature Metadata

label_name: psaM
feature_type: gene
region: Genome
length_bp: 93
variable_sites: 6
pi: 0.0394
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaM_p1	CCAGGCCAAGAAAAGCAGAA	ACTCATTGGGGAGGGAATGA	50.0	50.0	58.7	58.0	141-144 bp	1.0	79.6
psaM_p2	CCAGGCCAAGAAAAGCAGAA	TCAAACTCATTGGGGAGGGA	50.0	50.0	58.7	58.2	145-148 bp	1.0	77.6
psaM_p3	CCAGGCCAAGAAAAGCAGA	ACTCATTGGGGAGGGAATGA	52.6	50.0	58.0	58.0	141-144 bp	1.0	76.6
psaM_p4	CAGGCCAAGAAAAGCAGAAA	ACTCATTGGGGAGGGAATGA	45.0	50.0	56.8	58.0	140-143 bp	1.0	75.5
psaM_p5	CCAGGCCAAGAAAAGCAGAA	CAAACTCATTGGGGAGGGAA	50.0	50.0	58.7	57.4	144-147 bp	1.0	74.8