Hotspot

trnE-UUC-clpP

Genome · 29019-29641 · 623 bp

Back to Pinus

Pi 0.00241
MarkerSeek score 48.20
Primer available yes
Label trnE-UUC-clpP

Feature Metadata

label_name
trnE-UUC-clpP
feature_type
igs
region
Genome
length_bp
623
variable_sites
2
pi
0.00241
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-clpP_p1 ATCCACTAGACGATAGGGGC GGCACTTTTGGAACACCAAC 55.0 50.0 58.1 58.1 642-716 bp 1.0 82.4
trnE-UUC-clpP_p2 ATCCACTAGACGATAGGGGC AGGCACTTTTGGAACACCAA 55.0 45.0 58.1 58.1 643-717 bp 1.0 81.8
trnE-UUC-clpP_p3 CGTTGCCTCCTTGAAAGAGA GGCACTTTTGGAACACCAAC 50.0 50.0 57.8 58.1 671-745 bp 1.0 81.5
trnE-UUC-clpP_p4 CGAATGGATCATGGTACCCC GGCACTTTTGGAACACCAAC 55.0 50.0 57.8 58.1 709-783 bp 1.0 81.2
trnE-UUC-clpP_p5 ATCCACTAGACGATAGGGGC TCGACCCAAGTTGCATCTTC 55.0 50.0 58.1 58.2 681-755 bp 1.0 81.2