Hotspot

petN-psbM

Genome · 26939-27833 · 895 bp

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Pi 0.00168
MarkerSeek score 51.30
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
Genome
length_bp
895
variable_sites
3
pi
0.00168
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 CGGTATAACTTGGGCTGCTT GCAAGCAGTGGAAGCAATTG 50.0 50.0 58.0 58.6 989-995 bp 1.0 75.8
petN-psbM_p2 GGGGGAGAAGTGGGCTATAA GCAAGCAGTGGAAGCAATTG 55.0 50.0 58.2 58.6 930-936 bp 1.0 74.7
petN-psbM_p3 ACTTGGGCTGCTTTAATGGT GCAAGCAGTGGAAGCAATTG 45.0 50.0 57.7 58.6 982-988 bp 1.0 73.7
petN-psbM_p4 CACTCGTAGTATGGGGGAGA GCAAGCAGTGGAAGCAATTG 55.0 50.0 57.6 58.6 942-948 bp 1.0 73.3
petN-psbM_p5 TCACTCGTAGTATGGGGGAG GCAAGCAGTGGAAGCAATTG 55.0 50.0 57.6 58.6 943-949 bp 1.0 73.3