Hotspot

atpH-atpI

Genome · 13622-14312 · 691 bp

Back to Pinus

Pi 0.00289
MarkerSeek score 45.70
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
Genome
length_bp
691
variable_sites
4
pi
0.00289
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AATGGAAGCAAGCCCTACAG TCTTTGCAACTCTAGCTGCA 50.0 45.0 57.9 57.5 813 bp 1.0 74.9
atpH-atpI_p2 CAATGGAAGCAAGCCCTACA TCTTTGCAACTCTAGCTGCA 50.0 45.0 58.2 57.5 814 bp 1.0 74.7
atpH-atpI_p3 AATGGAAGCAAGCCCTACAG TAGGTGAATCCATGGAGGGT 50.0 50.0 57.9 57.4 786 bp 1.0 74.6
atpH-atpI_p4 CAATGGAAGCAAGCCCTACA TAGGTGAATCCATGGAGGGT 50.0 50.0 58.2 57.4 787 bp 1.0 74.4
atpH-atpI_p5 TCCAGCAGCAATAACGGAAG TCTTTGCAACTCTAGCTGCA 50.0 45.0 58.3 57.5 789 bp 1.0 73.9