Hotspot

psaM-ycf12

Genome · 8392-8684 · 293 bp

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Pi 0
MarkerSeek score 19.10
Primer available yes
Label psaM-ycf12

Feature Metadata

label_name
psaM-ycf12
feature_type
igs
region
Genome
length_bp
293
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
19.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaM-ycf12_p1 TCCGTAGAAAGATTCGGGGT CTAAAGGGCCCGATACAACC 50.0 55.0 58.1 58.0 813-816 bp 1.0 79.7
psaM-ycf12_p2 TCCGTAGAAAGATTCGGGGT AGCTATTCGGCTTCAACCTG 50.0 50.0 58.1 58.0 1324-1327 bp 1.0 79.4
psaM-ycf12_p3 TCCGTAGAAAGATTCGGGGT CCGCCACGATCTGATTGTAA 50.0 50.0 58.1 58.1 1004-1007 bp 1.0 79.4
psaM-ycf12_p4 TCCGTAGAAAGATTCGGGGT TGAGCTGCGCAATAACTTCT 50.0 45.0 58.1 57.9 777-780 bp 1.0 79.3
psaM-ycf12_p5 TCCGTAGAAAGATTCGGGGT TATACCCGCCACGATCTGAT 50.0 50.0 58.1 58.1 1009-1012 bp 1.0 79.2