Hotspot

ycf1

IRb · 106438-108042 · 1605 bp

Back to Pinguicula

Pi 0.0749

MarkerSeek score 67.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1605
variable_sites: 178
pi: 0.0749
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	TGAATTCCCGGTAGAAAGCG	55.0	50.0	58.0	58.0	2546 bp	0.333333	53.2
ycf1_p2	CCCCTTTGGTGTCATTGACA	TGAATTCCCGGTAGAAAGCG	50.0	50.0	58.0	58.0	2342 bp	0.333333	53.1
ycf1_p3	CCCGGAACTTCTTCGTAGTG	TGAATTCCCGGTAGAAAGCG	55.0	50.0	58.0	58.0	2451 bp	0.333333	53.1
ycf1_p4	CCGGAACTTCTTCGTAGTGG	TGAATTCCCGGTAGAAAGCG	55.0	50.0	58.0	58.0	2450 bp	0.333333	53.1
ycf1_p5	ATGTTTGCTCGCGACAATTG	TGAATTCCCGGTAGAAAGCG	45.0	50.0	58.0	58.0	2904 bp	0.333333	52.9